The Mausoleum of Ruhollah Khomeini (Persian: آرامگاه سید روح‌الله خمینی; Arabic: ضريح روح الله الخميني), also known as the Holy Shrine, or the Haram Motahhar, is a Shi'ite Islamic mausoleum that houses the tombs of Ayatollah Ruhollah Khomeini, his wife Khadijeh Saqafi, and his second son Ahmad Khomeini; and some political figures, such as former President Akbar Hashemi Rafsanjani, former Vice President Hassan Habibi, Lieutenant General Ali Sayad Shirazi, Iranian Revolution figure Sadeq Tabatabaei, and MP Marzieh Hadidchi. The mausoleum is located to the south of Tehran in the Behesht-e Zahra (Paradise of Zahra) cemetery.

The mausoleum is the centerpiece in a funerary complex spread over 2,000 hectares (4,900 acres), that houses the tombs, a cultural and tourist center, a university for Islamic studies, a seminary, a shopping mall, a 20,000-car park, and a branch of the National Museum of Iran. Construction commenced in 1989 following Khomeini's death on 3 June of that year, took over 35 years to complete, and the Iranian government reportedly devoted US$2 billion to the development. In May 2025 it was announced that the official commemoration will be held on 4 June 2025.

The site is a place of pilgrimage for followers of Khomeini. It is used symbolically by government figures, and is on occasion visited by foreign dignitaries. Every year, Khomeini's death anniversary is marked on 4 June at the mausoleum in a ceremony that is attended by governmental officials, foreign ambassadors, and others. Khomeini's grandson, Ayatollah Seyyed Hassan Khomeini, is in charge of caring for the mausoleum.