The Basilica of Saint Francis of Assisi (Italian: Basilica di San Francesco d'Assisi; Latin: Basilica Sancti Francisci Assisiensis) is the mother church of the Roman Catholic Order of Friars Minor Conventual in Assisi, a town in the Umbria region in central Italy, where Saint Francis was born and died. It is a papal minor basilica and one of the most important places of Christian pilgrimage in Italy. With its accompanying friary, Sacro Convento, the basilica is a distinctive landmark to those approaching Assisi. It has been a UNESCO World Heritage Site since 2000.

The basilica, which was begun in 1228, is built into the side of a hill and comprises two churches (known as the Upper Church and the Lower Church) and a crypt, where the remains of the saint are interred. The interior of the Upper Church is an important early example of the Gothic style in Italy. The Upper and Lower Churches are decorated with frescoes by numerous late medieval painters from the Roman and Tuscan schools, and include works by Cimabue, Giotto, Simone Martini, Pietro Lorenzetti, and possibly Pietro Cavallini. The range and quality of the works give the basilica a unique importance in demonstrating the outstanding development of Italian art of this period, especially if compared with the rest of Christian Europe.