Jacob's Ladder is a Grade I listed staircase leading from Jamestown, Saint Helena, up the side of Ladder Hill to Ladder Hill Fort. The name is a reference to the biblical Jacob's Ladder, a ladder extending to heaven.

The ladder is all that remains of a cable railway that was built there in the early 1800s. Its tracks and cars were later removed, although the stairs have remained in place and have become a tourist attraction connecting Jamestown and the suburb of Half Tree Hollow at the top of the hill.